mRNA distribution and membrane localization of the OAT-K1 organic anion transporter in rat renal tubules

Satohiro Masuda, Hideyuki Saito, Hiroshi Nonoguchi, Kimio Tomita, Ken Ichi Inui

研究成果: ジャーナルへの寄稿学術誌査読

92 被引用数 (Scopus)

抄録

OAT-K1, a renal organic anion transporter, which mediates methotrexate uptake, was analyzed for mRNA distribution along microdissected nephron segments and the immunolozalization in isolated plasma membranes from rat kidney. By using a reverse transcription-coupled PCR, OAT-K1 mRNA was detected predominantly in the superficial and juxtamedullary proximal straight tubules. Western blotting with antiserum for OAT-K1 revealed that the transporter protein with the apparent molecular mass of 40 kDa was expressed exclusively in the brush-border membranes from rat kidney. These findings suggest that the OAT-K1 is localized in the brush-border membranes of the renal proximal straight tubules.

本文言語英語
ページ(範囲)127-131
ページ数5
ジャーナルFEBS Letters
407
2
DOI
出版ステータス出版済み - 4月 28 1997
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

フィンガープリント

「mRNA distribution and membrane localization of the OAT-K1 organic anion transporter in rat renal tubules」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル