TY - JOUR
T1 - Mouse Embryonic Tooth Germ Dissection and Ex vivo Culture Protocol
AU - Han, Xue
AU - Yoshizaki, Keigo
AU - Tian, Tian
AU - Miyazaki, Kanako
AU - Takahashi, Ichiro
AU - Fukumoto, Satoshi
N1 - Funding Information:
This work was supported in part by a grants-in-aid for Scientific Research (18H03012, 17K19765, 15H05688 to K. Y.; 16H07065 to K. M. and 17H01606 to S. F.). This protocol was originally published in J Biol Chem (Han et al. 2018).
Publisher Copyright:
Copyright © 2020 The Authors; exclusive licensee Bio-protocol LLC.
PY - 2020/2/5
Y1 - 2020/2/5
N2 - A tooth germ ex vivo organ culture allows visualization of its development in different stages, thus enabling investigation of the molecular mechanisms of regulatory factors. Tooth germs can be rapidly dissected from E13 mouse embryos and placed on cell culture inserts for observation of subsequent tooth germ development in a three-dimensional situation in real time. This method is also suitable for other organs that develop by epithelial-mesenchymal interactions, including salivary gland, hair, lung, and kidney. In addition, siRNAs or growth factors can be easily added to ex vivo tooth germ cultures to investigate the detailed molecular function of specific genes. The present protocol provides an efficient and practical method for isolation and ex vivo culture of embryonic tooth germs.
AB - A tooth germ ex vivo organ culture allows visualization of its development in different stages, thus enabling investigation of the molecular mechanisms of regulatory factors. Tooth germs can be rapidly dissected from E13 mouse embryos and placed on cell culture inserts for observation of subsequent tooth germ development in a three-dimensional situation in real time. This method is also suitable for other organs that develop by epithelial-mesenchymal interactions, including salivary gland, hair, lung, and kidney. In addition, siRNAs or growth factors can be easily added to ex vivo tooth germ cultures to investigate the detailed molecular function of specific genes. The present protocol provides an efficient and practical method for isolation and ex vivo culture of embryonic tooth germs.
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U2 - 10.21769/BioProtoc.3515
DO - 10.21769/BioProtoc.3515
M3 - Article
AN - SCOPUS:85125452587
SN - 2331-8325
VL - 10
JO - Bio-protocol
JF - Bio-protocol
IS - 3
M1 - e3515
ER -