@inbook{060d9b38b24a40b2bd74caeb30a8ca55,
title = "Monitoring RhoGDI extraction of lipid-modified Rho GTPases from membranes using click chemistry",
abstract = "The posttranslational lipid modification of Rho GTPases is important for their proper subcellular localization and signal transduction. Rho GTPases terminate in a CaaX motif, in which the cysteine residue is modified with either a farnesyl or geranylgeranyl isoprenoid. RhoGDI renders Rho GTPases soluble by masking their lipid moieties. We recently identified that the brain-specific splice variant of Cdc42 (bCdc42) containing a noncanonical CCaX motif harbors a dual prenyl–palmitoyl modification that prevents its binding to RhoGDI. This chapter describes a method to analyze RhoGDI extraction of Rho GTPases containing different lipid modifications from membranes using a liposome reconstitution assay and click chemistry.",
author = "Akiyuki Nishimura and Linder, {Maurine E.}",
note = "Funding Information: This work was supported by the National Institutes of Health (R01 GM121540). Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",
year = "2019",
doi = "10.1007/978-1-4939-9532-5_22",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "297--306",
booktitle = "Methods in Molecular Biology",
}