TY - JOUR
T1 - Molecular cloning of mouse choline kinase and choline/ethanolamine kinase
T2 - Their sequence comparison to the respective rat homologs
AU - Aoyama, Chieko
AU - Nakashima, Kinichi
AU - Ishidate, Kozo
N1 - Funding Information:
This work was supported in part by a Grant-in-Aid (8672503) for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan. K.N. was supported by Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists. C.A. is a visiting graduate student from Kyoritsu College of Pharmacy, Japan.
PY - 1998/7/31
Y1 - 1998/7/31
N2 - Complementary DNAs homologous to a rat 42-kDa choline/ethanolamine kinase [C. Aoyama et al., Biochim. Biophys. Acta 1390 (1998) 1-7] and to a 50-kDa choline kinase [T. Uchida and S. Yamashita, J. Biol. Chem. 267 (1992) 10156-10162] were isolated from a 17-day post coitum mouse embryo cDNA library and their sequences were compared with the two murine species, respectively. The nucleotide sequence homology (within the coding sequence) between mouse and rat 50-kDa choline kinases (96.0%) was considerably higher than that between their 42-kDa choline/ethanolamine kinases (92.4%). Northern blot and RT-PCR studies on several rat tissues demonstrated that both isozymes are expressed ubiquitously with the highest level in testis. Copyright (C) 1998 Elsevier Science B.V.
AB - Complementary DNAs homologous to a rat 42-kDa choline/ethanolamine kinase [C. Aoyama et al., Biochim. Biophys. Acta 1390 (1998) 1-7] and to a 50-kDa choline kinase [T. Uchida and S. Yamashita, J. Biol. Chem. 267 (1992) 10156-10162] were isolated from a 17-day post coitum mouse embryo cDNA library and their sequences were compared with the two murine species, respectively. The nucleotide sequence homology (within the coding sequence) between mouse and rat 50-kDa choline kinases (96.0%) was considerably higher than that between their 42-kDa choline/ethanolamine kinases (92.4%). Northern blot and RT-PCR studies on several rat tissues demonstrated that both isozymes are expressed ubiquitously with the highest level in testis. Copyright (C) 1998 Elsevier Science B.V.
UR - http://www.scopus.com/inward/record.url?scp=0032584511&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032584511&partnerID=8YFLogxK
U2 - 10.1016/S0005-2760(98)00062-9
DO - 10.1016/S0005-2760(98)00062-9
M3 - Article
C2 - 9714798
AN - SCOPUS:0032584511
SN - 0005-2760
VL - 1393
SP - 179
EP - 185
JO - Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
JF - Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
IS - 1
ER -