Molecular biology of human angiotensin II receptor

Pharmacological studies have revealed the presence of two different subtypes (AT1 and AT2) for angiotensin II (Ang II) receptor. Recently, bovine and rat cDNAs of AT1 receptor were cloned by expression cloning. We have isolated a cDNA encoding human AT1 receptor from a human liver cDNA library, using rat AT1 receptor cDNA as a probe. Human AT1 receptor consists of 359 amino acid residues with a relative Mr of 41,060 and seven transmembrane segments, and was highly homologous to those of bovine, rat and mouse AT1 receptors. Southern blot analysis suggested that human AT1 receptor is a single copy gene.