Modulation of glutamate-induced outward current by prostaglandin E 2 in rat dissociated preoptic neurons

Toshihiko Katafuchi, Shumin Duan, Sachiko Take, Megumu Yoshimura

研究成果: ジャーナルへの寄稿学術誌査読

10 被引用数 (Scopus)


The preoptic/anterior hypothalamus (POA) is one of the major brain regions where cytokines and their related mediators (i.e., prostaglandins) exert diverse actions. In the present study, the modulatory effects of prostaglandin E 2 (PGE2) on the glutamate-induced membrane currents were examined using perforated-patch clamp method in rat POA neurons that had been mechanically dissociated by vibration without enzyme treatment. Application of glutamate through U-tube induced a slow outward current following fast inward ionotroic current at a holding membrane potential of -30 mV. The slow outward current was also induced by N-methyl-d-aspartate (NMDA), accompanied by an increased membrane conductance, and inhibited by perfusion with Ca 2+-free solution, tetraethylammonium chloride (TEA), and apamin, suggesting a Ca2+-dependent K+ current (KCa) activated by Ca2+ entry through NMDA channels. Perfusion with PGE2 at 0.1-10 μM, a principal mediator of fever and neuroendocrine control at the POA, did not produce apparent current by itself, but selectively potentiated the glutamate- or NMDA-induced KCa without affecting inward currents. The KCa induced by activation of NMDA receptors may serve as a feedback mechanism and the modulatory effects of PGE2 on the KCa may have an important physiological significance.

ジャーナルBrain Research
出版ステータス出版済み - 3月 10 2005

!!!All Science Journal Classification (ASJC) codes

  • 神経科学一般
  • 分子生物学
  • 臨床神経学
  • 発生生物学


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