TY - GEN
T1 - Microfluidic single-molecule nuclease digestion reveals rate-enhancing off-and-on molecular encountering function for site-specific DNA break
AU - Onoshima, D.
AU - Kaji, N.
AU - Tokeshi, M.
AU - Baba, Y.
PY - 2013
Y1 - 2013
N2 - An intermittent molecular encounter leading to a site-specific DNA-break was unveiled under a limited protein's sliding-free condition. It reflects a transient molecular action by which the reaction rate and efficiency of restriction enzyme in bacterial cells is enhanced. This was experimentally verified for the first time in the world by using our microfluidic device.
AB - An intermittent molecular encounter leading to a site-specific DNA-break was unveiled under a limited protein's sliding-free condition. It reflects a transient molecular action by which the reaction rate and efficiency of restriction enzyme in bacterial cells is enhanced. This was experimentally verified for the first time in the world by using our microfluidic device.
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UR - http://www.scopus.com/inward/citedby.url?scp=84907327883&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84907327883
SN - 9781632666246
T3 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
SP - 820
EP - 822
BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PB - Chemical and Biological Microsystems Society
T2 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Y2 - 27 October 2013 through 31 October 2013
ER -