Method for rapid distinction of Bombyx mandarina (Japan) from B. mandarina (China) based on rDNA sequence differences

Yuichi Kawanishi; Yutaka Banno; Hirofumi Fujimoto; Si Kab Nho; Zhenli Tu; Kazuei Mita; Kozo Tsuchida; Naoko Takada; Hideaki Maekawa; Yumiko Nakajima

Method for rapid distinction of Bombyx mandarina (Japan) from B. mandarina (China) based on rDNA sequence differences

Yuichi Kawanishi, Yutaka Banno, Hirofumi Fujimoto, Si Kab Nho, Zhenli Tu, Kazuei Mita, Kozo Tsuchida, Naoko Takada, Hideaki Maekawa, Yumiko Nakajima

システム生物工学

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

1 被引用数 (Scopus)

抄録

The EcoRI site is located between the 5.8S and 28S rRNA genes in Bombyx mandarina (Japan) (Japanese type rDNA) but is absent in the rDNA of B. mandarina (China)(B. mori type rDNA) including the domesticated B. mori. Digestion of PCR products amplified by primers of both flanking sides of this EcoRI site with EcoRI should only be successful for amplification products of B. mandarina (Japan) containing Japanese type rDNA. The distribution of Japanese type rDNA in Japan, Korea, and China was determined using this rapid detection method. Japanese type was not detected in China or in Korea despite having 27 chromosomes. These findings suggest that the choromosome fusion and the rDNA type separation were independent evolutionary events. Invasion of B. mori chromosome #11 into Japanese B. mandarina (Japan) was examined.

本文言語	英語
ページ（範囲）	79-85
ページ数	7
ジャーナル	Journal of Insect Biotechnology and Sericology
巻	77
号	2
出版ステータス	出版済み - 6月 1 2008

!!!All Science Journal Classification (ASJC) codes

バイオテクノロジー
応用微生物学とバイオテクノロジー
ビジネス、管理および会計（全般）
農業および生物科学（全般）
昆虫科学
産業および生産工学

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引用スタイル

@article{2bdc94fa41e4455a81273233a0d97b4f,

title = "Method for rapid distinction of Bombyx mandarina (Japan) from B. mandarina (China) based on rDNA sequence differences",

abstract = "The EcoRI site is located between the 5.8S and 28S rRNA genes in Bombyx mandarina (Japan) (Japanese type rDNA) but is absent in the rDNA of B. mandarina (China)(B. mori type rDNA) including the domesticated B. mori. Digestion of PCR products amplified by primers of both flanking sides of this EcoRI site with EcoRI should only be successful for amplification products of B. mandarina (Japan) containing Japanese type rDNA. The distribution of Japanese type rDNA in Japan, Korea, and China was determined using this rapid detection method. Japanese type was not detected in China or in Korea despite having 27 chromosomes. These findings suggest that the choromosome fusion and the rDNA type separation were independent evolutionary events. Invasion of B. mori chromosome #11 into Japanese B. mandarina (Japan) was examined.",

author = "Yuichi Kawanishi and Yutaka Banno and Hirofumi Fujimoto and Nho, {Si Kab} and Zhenli Tu and Kazuei Mita and Kozo Tsuchida and Naoko Takada and Hideaki Maekawa and Yumiko Nakajima",

year = "2008",

month = jun,

day = "1",

language = "English",

volume = "77",

pages = "79--85",

journal = "Journal of Insect Biotechnology and Sericology",

issn = "1346-8073",

publisher = "Japanese Society of Sericultural Sciences",

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TY - JOUR

T1 - Method for rapid distinction of Bombyx mandarina (Japan) from B. mandarina (China) based on rDNA sequence differences

AU - Kawanishi, Yuichi

AU - Banno, Yutaka

AU - Fujimoto, Hirofumi

AU - Nho, Si Kab

AU - Tu, Zhenli

AU - Mita, Kazuei

AU - Tsuchida, Kozo

AU - Takada, Naoko

AU - Maekawa, Hideaki

AU - Nakajima, Yumiko

PY - 2008/6/1

Y1 - 2008/6/1

N2 - The EcoRI site is located between the 5.8S and 28S rRNA genes in Bombyx mandarina (Japan) (Japanese type rDNA) but is absent in the rDNA of B. mandarina (China)(B. mori type rDNA) including the domesticated B. mori. Digestion of PCR products amplified by primers of both flanking sides of this EcoRI site with EcoRI should only be successful for amplification products of B. mandarina (Japan) containing Japanese type rDNA. The distribution of Japanese type rDNA in Japan, Korea, and China was determined using this rapid detection method. Japanese type was not detected in China or in Korea despite having 27 chromosomes. These findings suggest that the choromosome fusion and the rDNA type separation were independent evolutionary events. Invasion of B. mori chromosome #11 into Japanese B. mandarina (Japan) was examined.

AB - The EcoRI site is located between the 5.8S and 28S rRNA genes in Bombyx mandarina (Japan) (Japanese type rDNA) but is absent in the rDNA of B. mandarina (China)(B. mori type rDNA) including the domesticated B. mori. Digestion of PCR products amplified by primers of both flanking sides of this EcoRI site with EcoRI should only be successful for amplification products of B. mandarina (Japan) containing Japanese type rDNA. The distribution of Japanese type rDNA in Japan, Korea, and China was determined using this rapid detection method. Japanese type was not detected in China or in Korea despite having 27 chromosomes. These findings suggest that the choromosome fusion and the rDNA type separation were independent evolutionary events. Invasion of B. mori chromosome #11 into Japanese B. mandarina (Japan) was examined.

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M3 - Article

AN - SCOPUS:48849092361

SN - 1346-8073

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SP - 79

EP - 85

JO - Journal of Insect Biotechnology and Sericology

JF - Journal of Insect Biotechnology and Sericology

IS - 2

ER -

Method for rapid distinction of Bombyx mandarina (Japan) from B. mandarina (China) based on rDNA sequence differences

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!!!All Science Journal Classification (ASJC) codes

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