Mast cell maturation is driven via a group III phospholipase A ₂-prostaglandin D₂-DP1 receptor paracrine axis

Microenvironment-based alterations in phenotypes of mast cells influence the susceptibility to anaphylaxis, yet the mechanisms underlying proper maturation of mast cells toward an anaphylaxis-sensitive phenotype are incompletely understood. Here we report that PLA2G3, a mammalian homolog of anaphylactic bee venom phospholipase A₂, regulates this process. PLA2G3 secreted from mast cells is coupled with fibroblastic lipocalin-type PGD₂ synthase (L-PGDS) to provide PGD₂, which facilitates mast-cell maturation via PGD₂ receptor DP1. Mice lacking PLA2G3, L-PGDS or DP1, mast cell-deficient mice reconstituted with PLA2G3-null or DP1-null mast cells, or mast cells cultured with L-PGDS-ablated fibroblasts exhibited impaired maturation and anaphylaxis of mast cells. Thus, we describe a lipid-driven PLA2G3-L-PGDS-DP1 loop that drives mast cell maturation.