Mapping the origin and fate of myeloid cells in distinct compartments of the eye by single-cell profiling

Peter Wieghofer, Nora Hagemeyer, Roman Sankowski, Anja Schlecht, Ori Staszewski, Lukas Amann, Markus Gruber, Jana Koch, Annika Hausmann, Peipei Zhang, Stefaniya Boneva, Takahiro Masuda, Ingo Hilgendorf, Tobias Goldmann, Chotima Böttcher, Josef Priller, Fabio M.V. Rossi, Clemens Lange, Marco Prinz

研究成果: ジャーナルへの寄稿学術誌査読

62 被引用数 (Scopus)

抄録

Similar to the brain, the eye is considered an immune-privileged organ where tissue-resident macrophages provide the major immune cell constituents. However, little is known about spatially restricted macrophage subsets within different eye compartments with regard to their origin, function, and fate during health and disease. Here, we combined single-cell analysis, fate mapping, parabiosis, and computational modeling to comprehensively examine myeloid subsets in distinct parts of the eye during homeostasis. This approach allowed us to identify myeloid subsets displaying diverse transcriptional states. During choroidal neovascularization, a typical hallmark of neovascular age-related macular degeneration (AMD), we recognized disease-specific macrophage subpopulations with distinct molecular signatures. Our results highlight the heterogeneity of myeloid subsets and their dynamics in the eye that provide new insights into the innate immune system in this organ which may offer new therapeutic targets for ophthalmological diseases.

本文言語英語
論文番号e105123
ジャーナルEMBO Journal
40
6
DOI
出版ステータス出版済み - 3月 15 2021
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 神経科学一般
  • 分子生物学
  • 生化学、遺伝学、分子生物学一般
  • 免疫学および微生物学一般

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