@article{00e2e7e4f47f494ba5bda83633c0a669,
title = "Map-based cloning and characterization of a brown planthopper resistance gene BPH26 from Oryza sativa L. ssp. indica cultivar ADR52",
abstract = "The brown planthopper (BPH) is the most serious insect pest of rice in Asia. The indica rice cultivar ADR52 carries two BPH resistance genes, BPH26 (BROWN PLANTHOPPER RESISTANCE 26) and BPH25. Map-based cloning of BPH26 revealed that BPH26 encodes a coiled-coil-nucleotide-binding-site-leucine-rich repeat (CC-NBS-LRR) protein. BPH26 mediated sucking inhibition in the phloem sieve element. BPH26 was identical to BPH2 on the basis of DNA sequence analysis and feeding ability of the BPH2-virulent biotype of BPH. BPH2 was widely incorporated in elite rice cultivars and was well-cultivated in many Asian countries as a favorable gene resource in rice breeding against BPH. However, BPH2 was rendered ineffective by a virulent biotype of BPH in rice fields in Asia. In this study, we suggest that BPH2 can be reused by combining with other BPH resistance genes, such as BPH25, to ensure durable resistance to BPH.",
author = "Yasumori Tamura and Makoto Hattori and Hirofumi Yoshioka and Miki Yoshioka and Akira Takahashi and Jianzhong Wu and Naoki Sentoku and Hideshi Yasui",
note = "Funding Information: We thank Yano, M. for helpful advice and technical support; Kimura, Y., Hashino, K., Shiba, J. & Watanabe, M. for laboratory assistant; Hasegawa, T., Matsumoto, Y., Asano, T. & Kobayashi, T. for helpful discussion; Tagiri, A. & Hara, N. for technical support on in situ hybridization analysis; Ando, T. for technical support on molecular analysis; Sugimoto, K. & Ono, K. for helpful advice on plant transformation technics; Uchiyama, T. & Nojiri, M. in Hokko Chemical Industry co. Ltd for giving BPH colony collected in 1972; Yoshimura, A. & Yamagata, Y. for supporting material development. This work was supported by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Genomics for Agricultural Innovation QTL-2001 and Genomics-based Technology for Agricultural Improvement LCT-0011).",
year = "2014",
month = jul,
day = "29",
doi = "10.1038/srep05872",
language = "English",
volume = "4",
journal = "Scientific reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
}