TY - JOUR
T1 - Loss of CDCP1 Expression Promotes Invasiveness and Poor Prognosis in Esophageal Squamous Cell Carcinoma
AU - Sawada, Genta
AU - Takahashi, Yusuke
AU - Niida, Atsushi
AU - Shimamura, Teppei
AU - Kurashige, Junji
AU - Matsumura, Tae
AU - Ueo, Hiroki
AU - Uchi, Ryutaro
AU - Takano, Yuki
AU - Ueda, Masami
AU - Hirata, Hidenari
AU - Sakimura, Shotaro
AU - Shinden, Yoshiaki
AU - Eguchi, Hidetoshi
AU - Sudo, Tomoya
AU - Sugimachi, Keishi
AU - Miyano, Satoru
AU - Doki, Yuichiro
AU - Mori, Masaki
AU - Mimori, Koshi
N1 - Funding Information:
ACKNOWLEDGMENT This work was supported by the following Grants and foundations: Grants-in-Aid for Scientific Research (21229015); Funding Program for Next Generation World-Leading Researchers (LS094). We thank T. Shimo-oka, M. Kasagi, T. Kohno, K. Oda, M. Aoyagi, and T. Kawano for their assistance with molecular biological procedures.
Publisher Copyright:
© 2014, Society of Surgical Oncology.
PY - 2014
Y1 - 2014
N2 - Background: Human CDCP1 gene, located on chromosome 3p21.3, is a transmembrane glycoprotein widely expressed in epithelial tissues, and its role in cancer remains to be understood.Methods: Using microarray profiles of gene expression and copy number data from 69 esophageal squamous cell carcinoma (ESCC) samples, we performed informatics analyses to reveal the significance of CDCP1 expression. We also performed migration and invasion assays of siRNA-targeted CDCP1-transfected cells and CDCP1-overexpressing cell in vitro. Moreover, we evaluated the clinical magnitude of CDCP1 expression in esophageal squamous cell cancer cases.Results: Allelic loss of chromosome 3p was confirmed by copy number analysis. The expression level of CDCP1 in tumor tissue was significantly lower than that in corresponding normal tissue. siRNA targeting of CDCP1 promoted the migratory and invasive abilities of esophageal cancer cell lines, whereas both abilities were reduced in CDCP1-overexpressing cells. Gene set enrichment analysis showed that expression levels of CDCP1 were associated with tumor differentiation and metastasis, consistent with the result of clinicopathologic analyses. Finally, multivariate analysis revealed that the expression level of CDCP1 was an independent prognostic factor for survival.Conclusions: Loss of CDCP1 expression may be a novel indicator for biological aggressiveness in ESCC.
AB - Background: Human CDCP1 gene, located on chromosome 3p21.3, is a transmembrane glycoprotein widely expressed in epithelial tissues, and its role in cancer remains to be understood.Methods: Using microarray profiles of gene expression and copy number data from 69 esophageal squamous cell carcinoma (ESCC) samples, we performed informatics analyses to reveal the significance of CDCP1 expression. We also performed migration and invasion assays of siRNA-targeted CDCP1-transfected cells and CDCP1-overexpressing cell in vitro. Moreover, we evaluated the clinical magnitude of CDCP1 expression in esophageal squamous cell cancer cases.Results: Allelic loss of chromosome 3p was confirmed by copy number analysis. The expression level of CDCP1 in tumor tissue was significantly lower than that in corresponding normal tissue. siRNA targeting of CDCP1 promoted the migratory and invasive abilities of esophageal cancer cell lines, whereas both abilities were reduced in CDCP1-overexpressing cells. Gene set enrichment analysis showed that expression levels of CDCP1 were associated with tumor differentiation and metastasis, consistent with the result of clinicopathologic analyses. Finally, multivariate analysis revealed that the expression level of CDCP1 was an independent prognostic factor for survival.Conclusions: Loss of CDCP1 expression may be a novel indicator for biological aggressiveness in ESCC.
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U2 - 10.1245/s10434-014-3740-4
DO - 10.1245/s10434-014-3740-4
M3 - Article
C2 - 24849519
AN - SCOPUS:84939887929
SN - 1068-9265
VL - 21
SP - 640
EP - 647
JO - Annals of Surgical Oncology
JF - Annals of Surgical Oncology
IS - 4
ER -
