Localization of vascular endothelial growth factor in synovial membrane mast cells: Examination with 'multi-labelling subtraction immunostaining'

Takahiro Yamada, Motohiro Sawatsubashi, Hiroyuki Yakushiji, Yumi Itoh, Genichiro Edakuni, Michito Mori, Ladias Robert, Kohji Miyazaki

研究成果: ジャーナルへの寄稿学術誌査読

32 被引用数 (Scopus)

抄録

Mast cells are believed to play a novel part in the development of destructive synovial pannus in rheumatoid arthritis (RA). This study was undertaken to investigate the localization of vascular endothelial growth factor (VEGF) in the synovial membrane using a unique immunostaining technique. Synovial specimens of RA patients were examined immunohistochemically and were compared with specimens from non-RA controls. Multi-labelling subtraction immunostaining, a modification of double- and triple-labelling immunostaining, revealed that the VEGF-positive cells were identical to tryptase-positive cells (mast cells). No other cell types were found to be positive for VEGF. The synovium of RA patients showed a larger number of VEGF-positive mast cells than that of non-RA controls (P < 0.001). The study suggests that mast cell-derived VEGF may contribute to the development of synovial pannus in RA.

本文言語英語
ページ(範囲)567-570
ページ数4
ジャーナルVirchows Archiv
433
6
DOI
出版ステータス出版済み - 1998
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 病理学および法医学
  • 分子生物学
  • 細胞生物学

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