Live-cell metabolic analyzer protocol for measuring glucose and lactate metabolic changes in human cells

Kenji Miki; Mikako Yagi; Ko Igami; Hiroki Kittaka; Akito Tani; Natsuki Horiuchi; Satoshi Fukumoto; Koji Yoshimoto; Takeshi Uchiumi

doi:10.1016/j.xpro.2024.103518

Live-cell metabolic analyzer protocol for measuring glucose and lactate metabolic changes in human cells

Kenji Miki, Mikako Yagi, Ko Igami, Hiroki Kittaka, Akito Tani, Natsuki Horiuchi, Satoshi Fukumoto, Koji Yoshimoto, Takeshi Uchiumi

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

抄録

Understanding metabolic conditions related to glycolysis dependence is crucial for developing new treatments in cancer and regenerative medicine. This protocol details a method for using the live-cell metabolic analyzer (LiCellMo) to measure continuous changes in glucose consumption and lactate production in cultured human cells. LiCellMo provides real-time data on consecutive metabolic changes, improving measurements of these processes in various contexts, including in cancer and regenerative treatments.

本文言語	英語
論文番号	103518
ジャーナル	STAR Protocols
巻	6
号	1
DOI	https://doi.org/10.1016/j.xpro.2024.103518
出版ステータス	出版済み - 3月 21 2025

!!!All Science Journal Classification (ASJC) codes

神経科学一般
生化学、遺伝学、分子生物学一般
免疫学および微生物学一般

UN SDG

この成果は、次の持続可能な開発目標に貢献しています

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10.1016/j.xpro.2024.103518

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title = "Live-cell metabolic analyzer protocol for measuring glucose and lactate metabolic changes in human cells",

abstract = "Understanding metabolic conditions related to glycolysis dependence is crucial for developing new treatments in cancer and regenerative medicine. This protocol details a method for using the live-cell metabolic analyzer (LiCellMo) to measure continuous changes in glucose consumption and lactate production in cultured human cells. LiCellMo provides real-time data on consecutive metabolic changes, improving measurements of these processes in various contexts, including in cancer and regenerative treatments.",

keywords = "cell biology, cell culture, metabolism",

author = "Kenji Miki and Mikako Yagi and Ko Igami and Hiroki Kittaka and Akito Tani and Natsuki Horiuchi and Satoshi Fukumoto and Koji Yoshimoto and Takeshi Uchiumi",

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doi = "10.1016/j.xpro.2024.103518",

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T1 - Live-cell metabolic analyzer protocol for measuring glucose and lactate metabolic changes in human cells

AU - Miki, Kenji

AU - Yagi, Mikako

AU - Igami, Ko

AU - Kittaka, Hiroki

AU - Tani, Akito

AU - Horiuchi, Natsuki

AU - Fukumoto, Satoshi

AU - Yoshimoto, Koji

AU - Uchiumi, Takeshi

PY - 2025/3/21

Y1 - 2025/3/21

N2 - Understanding metabolic conditions related to glycolysis dependence is crucial for developing new treatments in cancer and regenerative medicine. This protocol details a method for using the live-cell metabolic analyzer (LiCellMo) to measure continuous changes in glucose consumption and lactate production in cultured human cells. LiCellMo provides real-time data on consecutive metabolic changes, improving measurements of these processes in various contexts, including in cancer and regenerative treatments.

AB - Understanding metabolic conditions related to glycolysis dependence is crucial for developing new treatments in cancer and regenerative medicine. This protocol details a method for using the live-cell metabolic analyzer (LiCellMo) to measure continuous changes in glucose consumption and lactate production in cultured human cells. LiCellMo provides real-time data on consecutive metabolic changes, improving measurements of these processes in various contexts, including in cancer and regenerative treatments.

KW - cell biology

KW - cell culture

KW - metabolism

UR - http://www.scopus.com/inward/record.url?scp=85214488334&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85214488334&partnerID=8YFLogxK

U2 - 10.1016/j.xpro.2024.103518

DO - 10.1016/j.xpro.2024.103518

M3 - Article

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AN - SCOPUS:85214488334

SN - 2666-1667

VL - 6

JO - STAR Protocols

JF - STAR Protocols

IS - 1

M1 - 103518

ER -

Live-cell metabolic analyzer protocol for measuring glucose and lactate metabolic changes in human cells

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!!!All Science Journal Classification (ASJC) codes

UN SDG

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