Liberation of oligosaccharides from glycosphingolipids on PC12 cell surface with endoglycoceramidase

Maija Liisa Rasilo, Makoto Ito, Tatsuya Yamagata

研究成果: ジャーナルへの寄稿学術誌査読

9 被引用数 (Scopus)

抄録

Endoglycoceramidase (EGCase) cleaves the linkage between the oligosaccharide and ceramide of glycosphingolipids (Ito, M., and Yamagata, T., J. Biol. Chem. 261, 14278-14282, ibid, 264, in press). Intact cells of rat pheochromocytoma line PC12 were treated with the highly purified EGCase I and the oligosaccharides released were analyzed by HPLC. Cleavage of the oligosaccharides with the enzyme reached a plateau as the amount of the enzyme was increased. At maximum, 42% of the oligosaccharides from globoside, 40% from GalGb3Cer, and 60% from Gb3Cer were liberated during 1h of incubation without impairing the viability of cells. The only partial liberation indicates that not all oligosaccharides of cell surface glycosphingolipids are accessible to the enzyme. The use of EGCase offers an important new method to access the functions of glycosphingolipids on cell surface in situ.

本文言語英語
ページ(範囲)1093-1099
ページ数7
ジャーナルBiochemical and Biophysical Research Communications
162
3
DOI
出版ステータス出版済み - 8月 15 1989
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「Liberation of oligosaccharides from glycosphingolipids on PC12 cell surface with endoglycoceramidase」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル