TY - JOUR
T1 - Isolation and characterization of tetranucleotide microsatellite markers for Pinus merkusii
AU - Thao, D. V.
AU - Widyatmoko, A. Y.P.B.C.
AU - Guan, L.
AU - Gotoh, E.
AU - Watanabe, A.
AU - Shiraishi, S.
N1 - Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2013/6
Y1 - 2013/6
N2 - Pinus merkusii is an important tree native to Southeast Asia. Thirteen tetranucleotide microsatellite makers were developed from this species using a dual-suppression polymerase chain reaction technique. Of these markers, nine loci were polymorphic. The mean number of alleles per locus was 3. 18 (range 2-5) and mean expected heterozygosities were 0. 525 (range 0. 384-0. 712). These microsatellite markers will be available to assess the genetic diversity, mating patterns and population structure which will facilitate conservation and management strategies.
AB - Pinus merkusii is an important tree native to Southeast Asia. Thirteen tetranucleotide microsatellite makers were developed from this species using a dual-suppression polymerase chain reaction technique. Of these markers, nine loci were polymorphic. The mean number of alleles per locus was 3. 18 (range 2-5) and mean expected heterozygosities were 0. 525 (range 0. 384-0. 712). These microsatellite markers will be available to assess the genetic diversity, mating patterns and population structure which will facilitate conservation and management strategies.
KW - Dual-suppression PCR
KW - Pinus merkusii
KW - Tetranucleotide microsatellite marker
UR - http://www.scopus.com/inward/record.url?scp=84876826830&partnerID=8YFLogxK
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U2 - 10.1007/s12686-012-9821-4
DO - 10.1007/s12686-012-9821-4
M3 - Article
AN - SCOPUS:84876826830
SN - 1877-7252
VL - 5
SP - 433
EP - 436
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
IS - 2
ER -