TY - JOUR
T1 - Isolation and characterization of alkane-utilizing Nocardioides sp. strain CF8
AU - Hamamura, Natsuko
AU - Arp, Daniel J.
N1 - Funding Information:
This work was supported by the National Institutes of Health Grant GM56128 to D.J.A. and by a research grant from the R2D2 program of the US EPA-sponsored Western Region Hazardous Substance Research Center under agreement R-815738 to L.S. and D.J.A.
PY - 2000/5/1
Y1 - 2000/5/1
N2 - A butane-utilizing bacterial strain CF8 was isolated and identified as a member of the genus Nocardioides from chemotaxonomic and 16S rDNA sequence analysis. Strain CF8 grew on alkanes ranging from C2 to C16 in addition to butane and various other substrates including primary alcohols, carboxylic acids, and phenol. Butane degradation by strain CF8 was inactivated by light, a specific inactivator of copper-containing monooxygenases. The unique thermal aggregation phenomenon of acetylene-binding polypeptides was also observed for strain CF8. These results suggest that butane monooxygenase in strain CF8 is a third example of the copper-containing monooxygenases previously described in ammonia oxidizers and methanotrophs. Copyright (C) 2000 Federation of European Microbiological Societies.
AB - A butane-utilizing bacterial strain CF8 was isolated and identified as a member of the genus Nocardioides from chemotaxonomic and 16S rDNA sequence analysis. Strain CF8 grew on alkanes ranging from C2 to C16 in addition to butane and various other substrates including primary alcohols, carboxylic acids, and phenol. Butane degradation by strain CF8 was inactivated by light, a specific inactivator of copper-containing monooxygenases. The unique thermal aggregation phenomenon of acetylene-binding polypeptides was also observed for strain CF8. These results suggest that butane monooxygenase in strain CF8 is a third example of the copper-containing monooxygenases previously described in ammonia oxidizers and methanotrophs. Copyright (C) 2000 Federation of European Microbiological Societies.
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U2 - 10.1016/S0378-1097(00)00109-9
DO - 10.1016/S0378-1097(00)00109-9
M3 - Article
C2 - 10779707
AN - SCOPUS:0034194873
SN - 0378-1097
VL - 186
SP - 21
EP - 26
JO - FEMS microbiology letters
JF - FEMS microbiology letters
IS - 1
ER -