Involvement of periostin in regression of hyaloidvascular system during ocular development

Mitsuru Arima, Shigeo Yoshida, Takahito Nakama, Keijiro Ishikawa, Shintaro Nakao, Takeru Yoshimura, Ryo Asato, Yukio Sassa, Takeshi Kita, Hiroshi Enaida, Yuji Oshima, Akira Matsuda, Akira Kudo, Tatsuro Ishibashi

研究成果: ジャーナルへの寄稿学術誌査読

18 被引用数 (Scopus)


Purpose. A timely regression of the hyaloid vascular system (HVS) is required for the normal ocular development. Although macrophages have a critical role in this process, the exact mechanism remains undetermined. Periostin is a matricellular protein involved in tissue and vascular remodeling. The purpose of our study was to determine whether periostin is involved in the HVS regression. Methods. We used wild type (WT) and periostin knockout (KO) mice. Indocyanine green angiography and immunohistochemistry with isolectin B4 were used to evaluate the HVS regression. TUNEL-labeling was used to quantify the number of apoptotic hyaloid vascular endothelial cells. F4/80 and Iba-1 staining was performed to determine the number and location of macrophages in the vitreous. The location of periostin also was investigated by immunohistochemistry. To determine the functional role of periostin, the degree of adhesion of human monocytes to fibronectin was measured by an adhesion assay. Results. The HVS regression and peak in the number of TUNEL-positive apoptotic endothelial cells were delayed in periostin KO mice. The number of F4/80 positive cells in the vitreous was higher in periostin KO mice. Only a small number of Iba-1-positive cells near the hyaloid vessels was co-stained with periostin, and peripheral blood monocytes were not stained with periostin. Adhesion assay showed that periostin increased the degree of attachment of monocytes to fibronectin. Conclusions. These results suggest that periostin, which is secreted by the intraocular macrophages, enhances the HVS regression by intensifying the adhesion of macrophages to hyaloid vessels.

ジャーナルInvestigative Ophthalmology and Visual Science
出版ステータス出版済み - 9月 2012

!!!All Science Journal Classification (ASJC) codes

  • 眼科学
  • 感覚系
  • 細胞および分子神経科学


「Involvement of periostin in regression of hyaloidvascular system during ocular development」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。