Involvement of M1/M2 Macrophage Polarization in Reparative Dentin Formation

Masataka Kadowaki, Shinichiro Yoshida, Tomohiro Itoyama, Atsushi Tomokiyo, Sayuri Hamano, Daigaku Hasegawa, Hideki Sugii, Hiroshi Kaneko, Risa Sugiura, Hidefumi Maeda

研究成果: ジャーナルへの寄稿学術誌査読

3 被引用数 (Scopus)


In cases in which dental pulp tissue is accidentally exposed, direct pulp capping is often performed to induce reparative dentin formation. Although macrophages are essential for the inflammatory response and tissue repair, the emergence pattern and the role of macrophages in dental pulp tissue have not been clarified. Here, we investigated the emergence of M1/M2 macrophages in dental pulp tissue after a direct pulp capping and the effects of M2 macrophages on odontoblastic differentiation of the dental pulp stem cell (DPSC) clones. The emergence of macrophages in dental pulp tissue was investigated using a rat direct pulp capping model. Alizarin Red S staining and quantitative RT-PCR was performed to examine the effect of M2 macrophages on the mineralization and odontoblastic differentiation of DPSC clones. Immunohistochemical staining revealed that M1 macrophages were detected in dental pulp tissue after treatment and increased in number at three days after treatment. However, M2 macrophages gradually increased in number in dental pulp tissue after treatment, with the highest level recorded at seven days post-operation. Additionally, conditioned medium from M2 macrophages induced odontoblast-like differentiation of DPSC clones. These results suggest that macrophages play a role in the inflammatory response and reparative dentin formation after dental pulp exposure.

出版ステータス出版済み - 11月 2022

!!!All Science Journal Classification (ASJC) codes

  • 生態、進化、行動および分類学
  • 生化学、遺伝学、分子生物学一般
  • 宇宙惑星科学
  • 古生物学


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