TY - JOUR
T1 - Involvement of intracellular cAMP in epirubicin-induced vascular endothelial cell injury
AU - Yamada, Takaaki
AU - Ueda, Mitsuyo
AU - Egashira, Nobuaki
AU - Zukeyama, Nina
AU - Kuwahara, Jun
AU - Masuda, Satohiro
N1 - Funding Information:
This study was supported by a grant from Fukuoka Foundation for Sound Health Cancer Research Fund and JSPS KAKENHI Grant Number 25460335 . We appreciate the technical support from the Research Support Center, Graduate School of Medical Sciences, Kyushu University, Fukuoka.
Publisher Copyright:
© 2016 Japanese Pharmacological Society.
PY - 2016/1/1
Y1 - 2016/1/1
N2 - We investigated the involvement of intracellular cAMP in endothelial cell injury induced by epirubicin. Epirubicin-induced decrease in cell viability and increase in caspase-3/7 activity were reversed by a cAMP analog dibutyryl cAMP (DBcAMP) or an activator of adenylate cyclase forskolin concomitant with a phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine. Moreover, epirubicin-induced elevation of lipid peroxide levels was attenuated by DBcAMP. Interestingly, the exposure of epirubicin decreased intracellular cAMP levels before the onset of epirubicin-induced production of lipid peroxidation. These results suggest that intracellular cAMP plays an important role in epirubicin-induced endothelial cell injury.
AB - We investigated the involvement of intracellular cAMP in endothelial cell injury induced by epirubicin. Epirubicin-induced decrease in cell viability and increase in caspase-3/7 activity were reversed by a cAMP analog dibutyryl cAMP (DBcAMP) or an activator of adenylate cyclase forskolin concomitant with a phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine. Moreover, epirubicin-induced elevation of lipid peroxide levels was attenuated by DBcAMP. Interestingly, the exposure of epirubicin decreased intracellular cAMP levels before the onset of epirubicin-induced production of lipid peroxidation. These results suggest that intracellular cAMP plays an important role in epirubicin-induced endothelial cell injury.
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U2 - 10.1016/j.jphs.2015.12.010
DO - 10.1016/j.jphs.2015.12.010
M3 - Article
C2 - 26809377
AN - SCOPUS:84957594442
SN - 1347-8613
VL - 130
SP - 33
EP - 37
JO - Journal of Pharmacological Sciences
JF - Journal of Pharmacological Sciences
IS - 1
ER -