Investigation of optimum culture condition for hybrid artificial liver module using PUF/porcine hepatocyte spheroid culture

K. Nakazawa, H. Ijima, M. Kaneko, T. Ito, T. Matsushita, T. Gion, K. Shirabe, M. Shimada, K. Takenaka, K. Sugimachi, K. Funatsu

研究成果: ジャーナルへの寄稿学術誌査読

5 被引用数 (Scopus)

抄録

In this study, we determined optimum culture conditions of primary porcine hepatocytes for development of hybrid artificial liver using polyurethane foam (PUF)/spheroid culture. Optimum components of culture medium were investigated in stationary culture using PUF plate (25 x 25 x 1 mm). Immobilized cell density and intracapillary medium velocity in multi- capillary (MC) PUF packed-bed module (volume: 18.8 cm3) were estimated in perfusion culture. As a result, porcine hepatocytes formed spheroid spontaneously and maintained ammonia metabolic activity in Williams'E medium supplemented with 10 to 20% FBS. And high activities of ammonia metabolism and albumin secretion were performed at the immobilized cell density. This study provided optimum culture condition for hybrid artificial liver module using PUF/porcine hepatocyte spheroid culture.

本文言語英語
ページ(範囲)222-226
ページ数5
ジャーナルJapanese Journal of Artificial Organs
27
1
出版ステータス出版済み - 1998

!!!All Science Journal Classification (ASJC) codes

  • 生物理学

フィンガープリント

「Investigation of optimum culture condition for hybrid artificial liver module using PUF/porcine hepatocyte spheroid culture」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル