Intraoperative real-time genetic diagnosis for sentinel node navigation surgery

H. Hamakawa, A. Onishi, T. Sumida, N. Terakado, S. Hino, K. I. Nakashiro, S. Shintani

研究成果: ジャーナルへの寄稿学術誌査読

22 被引用数 (Scopus)


Sentinel node navigation surgery (SNNS) has received considerable attention for its role in deciding whether to perform neck dissection in patients with early oral cancer. However, diagnostic accuracy and its intraoperative availability of results remain important concerns. First, we shortened the examination time required for genetic diagnosis. Second, we assessed the quality of the extracted mRNA. Third, 10 patients with early N0 oral cancer underwent SNNS, using our new technique for genetic diagnosis to determine whether neck dissection was required. The examination time of our one-step reverse-transcriptase polymerase chain reaction method using a minicolumn and LightCycler was successfully shortened to 2 h, permitting intraoperative genetic diagnosis. The extracted mRNA was of high quality. Six sentinel nodes in four patients were diagnosed to be metastatic on genetic diagnosis; these patients underwent neck dissection. The other six patients avoided unnecessary surgery. We conclude that intraoperative genetic diagnosis of micrometastasis holds promise of being a sensitive method that can be used to support SNNS.

ジャーナルInternational Journal of Oral and Maxillofacial Surgery
出版ステータス出版済み - 10月 2004

!!!All Science Journal Classification (ASJC) codes

  • 外科
  • 口腔外科
  • 耳鼻咽喉科学


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