Inhibitionof cell motility by fucoidan derived from Cladosiphon okamuranus

Fucoidanis anactive component of seaweed that has beenshownto exhibit a number of biological regulation effects, such as inhibition of proliferation and induction of apoptosis, invarious tumor cells. Inthis study, we demonstrated that fucoidanisolated from Cladosiphon okamuranus significantly inhibited cell motility in several cancer cells in a dose-dependent manner. Fucoidan induced the disruption of stress fibers at concentrations greater than 10 μg/mL. However, fucoidanwas not shownto decrease the phosphorylationof the myosinII regulatory light chain(MRLC) at Thr18/Ser19 inB16 cells. These data suggested that the inhibition of cell motility by treatment with fucoidan was induced via a pathway other than MRLC.