Inhibition of p600 expression suppresses both invasiveness and anoikis resistance of gastric cancer

Hiroshi Sakai, Kenoki Ohuchida, Kazuhiro Mizumoto, Lin Cui, Kohei Nakata, Hiroki Toma, Eishi Nagai, Masao Tanaka

研究成果: ジャーナルへの寄稿学術誌査読

7 被引用数 (Scopus)


Background. Advanced gastric cancers often metastasize to distant organs and the peritoneum, leading to a poor prognosis. Both invasiveness and resistance to anchorageindependent cell death (anoikis) are important factors in the process of metastasis. p600 (600-kDa protein), recently identified from a cervical cancer cell line, plays a role in both anoikis resistance and cell migration. In this study, we examined whether p600 is involved in the progression of gastric cancer. Methods. We used both normal gastric mucosal cells and cancer cells laser-microdissected from 42 gastric cancers and their normal counterparts, and compared their p600 mRNA expression levels with quantitative reverse transcriptase-polymerase chain reaction. We inhibited p600 expression in two gastric cancer cell lines with siRNA and examined its effect on the invasiveness and anoikis resistance both in vitro and in vivo. Results. Expression of p600 mRNA was significantly higher in gastric cancer cells than in normal mucosal cells (P = 0.027). The invasion assay revealed that invasiveness was significantly reduced by inhibition of p600 (P<0.01). In vitro experiments revealed that cell viability and colonyformation capacity under anchorage-independent conditions were significantly reduced by inhibition of p600 (P<0.05). In vivo experiments also showed that the establishment of intraperitoneal disseminated tumors was significantly suppressed by transient inhibition of p600 (P<0.001). Conclusions. Our results strongly suggest that p600 is involved in gastric cancer progression, and has a potential to be a new molecular target for gastric cancer therapy.

ジャーナルAnnals of Surgical Oncology
出版ステータス出版済み - 7月 2011

!!!All Science Journal Classification (ASJC) codes

  • 外科
  • 腫瘍学


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