TY - JOUR
T1 - Inhibition of leukotriene formation in human leukocytes by halothane
AU - Atsushi, Yamaoka
AU - Hideki, Sumimoto
AU - Koichiro, Takeshige
AU - Takeo, Itoh
AU - Ryuichi, Isobe
AU - Junichi, Yoshitake
AU - Shigeki, Minakami
N1 - Funding Information:
We thank Dr. Yuichi Kanmura (Department of Pharmacology, School of Medicine, Kyushu University) for the identification of leukotriene C, by bioassay. This study was supported in part by grants from the Ministry of Education, Science and Culture and the Ministry of Health and Welfare.
PY - 1987/4/24
Y1 - 1987/4/24
N2 - The effects of an inhalation anesthetic, halothane (2-bromo-2-chloro-1,1,1-trifluoroethane) on the formation of 5-lipoxygenase metabolites such as leukotriene B4, 5(S)-hydroxyeicosatetraenoic acid (5-HETE), 6-trans-isomers of leukotriene B4 and leukotriene C4 were studied in human leukocytes stimulated with calcium ionophore A23187. Halothane inhibited the formation of all these metabolites dose dependently and the formation was restored by removal of the drug. The anesthetic also reversibly inhibited the release of [3H]arachidonic acid from neutrophils with a half-inhibition concentration of less than 0.19 mM. The formation of 5-lipoxygenase metabolites was not inhibited by the anesthetic when leukocytes were stimulated with the ionophore in the presence of exogenous arachidonic acid. These observations indicate that the inhibitory effect of halothane on the formation of 5-lipoxygenase metabolites in leukocytes is mainly due to the inhibition of arachidonic acid release.
AB - The effects of an inhalation anesthetic, halothane (2-bromo-2-chloro-1,1,1-trifluoroethane) on the formation of 5-lipoxygenase metabolites such as leukotriene B4, 5(S)-hydroxyeicosatetraenoic acid (5-HETE), 6-trans-isomers of leukotriene B4 and leukotriene C4 were studied in human leukocytes stimulated with calcium ionophore A23187. Halothane inhibited the formation of all these metabolites dose dependently and the formation was restored by removal of the drug. The anesthetic also reversibly inhibited the release of [3H]arachidonic acid from neutrophils with a half-inhibition concentration of less than 0.19 mM. The formation of 5-lipoxygenase metabolites was not inhibited by the anesthetic when leukocytes were stimulated with the ionophore in the presence of exogenous arachidonic acid. These observations indicate that the inhibitory effect of halothane on the formation of 5-lipoxygenase metabolites in leukocytes is mainly due to the inhibition of arachidonic acid release.
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U2 - 10.1016/0005-2760(87)90232-3
DO - 10.1016/0005-2760(87)90232-3
M3 - Article
C2 - 3032266
AN - SCOPUS:0023251146
SN - 0005-2760
VL - 918
SP - 284
EP - 292
JO - Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
JF - Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
IS - 3
ER -