TY - JOUR
T1 - Inhibition by lithium of cyclic GMP formation without inhibition of nitric oxide generation in the mouse neuroblastoma cell (N1e-115)
AU - Shintani, Futoshi
AU - Kanba, Shigenobu
AU - Nakaki, Toshio
AU - Nakamura, Ryoko
AU - Sato, Koichi
AU - Yagi, Gohei
AU - Richelson, Elliott
AU - Kato, Ryuichi
AU - Asai, Masahiro
N1 - Funding Information:
This work was supported in part by Keio Gijuku Academic Development Funds, a Research Grant for Nervous and Mental Disorders from the Ministry of Health and Welfare and a Research Grant from Ohme Keiyu Hospital (Director: Nobuo Otsuka, M.D.).
Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1994/10
Y1 - 1994/10
N2 - We investigated the effects of lithium ion (Li+) on muscarinic receptor-mediated nitric oxide (NO) generation, and guanylate cyclase (GCase) activation using the mouse neuroblastoma clone, NIE-115. The levels of released NO were determined by measuring the levels of nitrite/nitrate in the incubation medium, and the activity of GCase was measured with an assay for cellular cyclic [3H] GMP levels. We determined that Li+ had no effects on muscarinic receptor-activated elevation of nitrite/nitrate levels, which were significantly inhibited by 100 11M L-NG-monomethylarginine, although it has been reported that Li+ inhibits muscarinic receptor- activated cyclic GMP formation in the cells. In addition, Li+ inhibited the cyclic GMP formation induced by an NO donor, sodium nitroprusside (SNP), in both intact cells and a crude cellular homogenate; thus, the inhibition by Li+ of muscarinic receptor-mediated cyclic GMP synthesis appeared to be at the level of GCase, but not NO synthase.
AB - We investigated the effects of lithium ion (Li+) on muscarinic receptor-mediated nitric oxide (NO) generation, and guanylate cyclase (GCase) activation using the mouse neuroblastoma clone, NIE-115. The levels of released NO were determined by measuring the levels of nitrite/nitrate in the incubation medium, and the activity of GCase was measured with an assay for cellular cyclic [3H] GMP levels. We determined that Li+ had no effects on muscarinic receptor-activated elevation of nitrite/nitrate levels, which were significantly inhibited by 100 11M L-NG-monomethylarginine, although it has been reported that Li+ inhibits muscarinic receptor- activated cyclic GMP formation in the cells. In addition, Li+ inhibited the cyclic GMP formation induced by an NO donor, sodium nitroprusside (SNP), in both intact cells and a crude cellular homogenate; thus, the inhibition by Li+ of muscarinic receptor-mediated cyclic GMP synthesis appeared to be at the level of GCase, but not NO synthase.
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U2 - 10.1038/npp.1994.41
DO - 10.1038/npp.1994.41
M3 - Article
C2 - 7840863
AN - SCOPUS:0028073483
SN - 0893-133X
VL - 11
SP - 119
EP - 124
JO - Neuropsychopharmacology
JF - Neuropsychopharmacology
IS - 2
ER -
