In vivo and in vitro interactions of the Bombyx mori chymotrypsin inhibitor b1 with Escherichia coli

Ningjia He, Yoichi Aso, Hiroshi Fujii, Yutaka Banno, Kohji Yamamoto

研究成果: ジャーナルへの寄稿学術誌査読

15 被引用数 (Scopus)

抄録

Various chymotrypsin inhibitors occur in the hemolymph of silkworm larvae. Interaction of chymotrypsin inhibitor b1 (CI-b1) with Escherichia coli was examined from the viewpoint of action against invading bacteria. Injection of dead E. coli cells into larva reduced the CI-b1 content of the hemolymph, suggesting in vivo binding of CI-b1 to the outer membrane of the cell. Results from incubation of E. coli in cell-free hemolymph in the presence or absence of lipopolysaccharide indicated that CI-b1 is the only CI bound to E. coli and that it interacts with lipopolysaccharide. CI-b1 formed a complex with lipopolysaccharide in vitro; the value of the dissociation constant was relatively large. Inhibitory activity of CI-b1 changed insignificantly in mixture with lipopolysaccharide. CI-b1 affected the growth of E. coli but never worked lethally. CI-b1 is speculated to be a mediator that scavenges intruding bacteria rather than a direct anti-bacterial factor. This is the first report confirming that CI-b1 is a lipopolysaccharide binding protein.

本文言語英語
ページ(範囲)835-840
ページ数6
ジャーナルBioscience, Biotechnology and Biochemistry
68
4
DOI
出版ステータス出版済み - 4月 2004

!!!All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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