In situ nick translation method reveals DNA strand scission in HeLa cells following heat treatment

Hideaki Anai, Yoshihiko Maehara, Keizo Sugimachi

研究成果: ジャーナルへの寄稿学術誌査読

30 被引用数 (Scopus)

抄録

DNA strand break in HeLa cells induced by heat was detected using the in situ nick translation method. The cells were incubated at 43°C for various times (15, 30, 45, 60, 90 or 120 min) in Lab-Tek tissue culture chamber/slides and were fixed with ethanol/acetic acid on the slide glass. The break sites in DNA were translated artificially in the presence of Escherichia coli DNA polymerase I and 3H-labeled dTTP. The level of break sites in the DNA was visualized by autoradiographic observation of the grains. The DNA strand break appeared as early as 15 min, increased to 10.3-fold at 45 min of 43°C treatment and this level related reciprocally to clonogenicity of the cell. The nick translation method thus provides a rapid in situ assay for determining heat-induced DNA damage of cultured cells, in a semi-quantitative manner.

本文言語英語
ページ(範囲)33-38
ページ数6
ジャーナルCancer Letters
40
1
DOI
出版ステータス出版済み - 5月 1988
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 腫瘍学
  • 癌研究

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