Identification of corresponding genes for three low-α-linolenic acid mutants and elucidation of their contribution to fatty acid biosynthesis in soybean seed

Toyoaki Anai, Tomoko Yamada, Takehito Kinoshita, Shaikh M. Rahman, Yutaka Takagi

研究成果: ジャーナルへの寄稿学術誌査読

58 被引用数 (Scopus)

抄録

In order to keep the quality of vegetable oil stable, it is important to reduce the α-linolenic acid content. Previously, we developed three low-α-linonenic acid soybean mutants, 'J18', 'M5', and 'M24'. In this study, we obtained and characterized four cDNAs, GmFAD3-1a, GmFAD3-1b, GmFAD3-2a, and GmFAD3-2b, encoding microsomal ω-3 fatty acid desaturase from soybean developing seeds. The GmFAD3-1b transcript does not accumulate in the 'J18' mutant, whereas 'M5' and 'M24' contain a 19-base pair and a single-base pair deletion in the coding region of the GmFAD3-1b and GmFAD3-1a genes, respectively. Furthermore, heterologous expression of these two mutant genes in Saccharomyces cerevisiae revealed that the COOH-terminal region of both mutant gene products is essential for their enzymatic activity. In this report, we also discuss the contribution of each microsomal ω-3 fatty acid desaturase gene for α-linolenic acid biosynthesis in soybean seeds.

本文言語英語
ページ(範囲)1615-1623
ページ数9
ジャーナルPlant Science
168
6
DOI
出版ステータス出版済み - 6月 2005
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 遺伝学
  • 農業および作物学
  • 植物科学

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