Identification of an imprinted U2af binding protein related sequence on mouse chromosome 11 using the RLGS method

Y. Hayashizaki, H. Shibata, S. Hirotsune, H. Sugino, Y. Okazaki, N. Sasaki, K. Hirose, H. Imoto, H. Okuizumi, M. Muramatsu, H. Komatsubara, T. Shiroishi, K. Moriwaki, M. Katsuki, N. Hatano, H. Sasaki, T. Ueda, N. Mise, N. Takagi, C. PlassV. M. Chapman

研究成果: ジャーナルへの寄稿学術誌査読

205 被引用数 (Scopus)

抄録

A new imprinted gene has been discovered in mice using the technique of restriction landmark genomic scanning (RGLS) with methylation sensitive enzymes. Eight out of 3,100 strain–specific Notl and BssHII spots were identified as imprinted in reciprocal F1 hybrids. Subsequently, we isolated a genomic clone for one locus on proximal chromosome 11 near the Glns locus, an imprinted region in uniparental disomic mice, and its corresponding cDNA clone. Expression of this transcript from the paternal allele was established using RT–PCR of reciprocal F1–hybrid mice. The amino–acid sequence deduced from the cDNA showed significant homology to the U2 small nuclear ribonucleoprotein auxiliary factor 35 kDa subunit.

本文言語英語
ページ(範囲)33-40
ページ数8
ジャーナルNature genetics
6
1
DOI
出版ステータス出版済み - 1月 1994
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 遺伝学

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