Identification of a new sprouty protein responsible for the inhibition of the Bombyx mori nucleopolyhedrovirus reproduction

Shengkai Jin, Tingcai Cheng, Liang Jiang, Ping Lin, Qiong Yang, Yang Xiao, Takahiro Kusakabe, Qingyou Xia

研究成果: ジャーナルへの寄稿学術誌査読

20 被引用数 (Scopus)

抄録

The rat sarcoma-extracellular signal regulated kinase mitogen-activated protein kinases pathway, one of the most ancient signaling pathways, is crucial for the defense against Bombyx mori nucleopolyhedrovirus (BmNPV) infection. Sprouty (Spry) proteins can inhibit the activity of this pathway by receptor tyrosine kinases. We cloned and identified a new B. mori gene with a Spry domain similar to the Spry proteins of other organisms, such as fruitfly, mouse, human, chicken, Xenopus and zebrafish, and named it BmSpry. The gene expression analysis showed that BmSpry was transcribed in all of the examined tissues and in all developmental stages from embryo to adult. BmSpry also induced expression of BmNPV in the cells. Our results indicated: (1) the knock-down of BmSpry led to increased BmNPV replication and silkworm larvae mortality; (2) overexpression of BmSpry led to reduced BmNPV replication; and (3) BmSpry regulated the activation of ERK and inhibited BmNPV replication. These results showed that BmSpry plays a crucial role in the antiviral defense of the silkworm both in vitro and in vivo.

本文言語英語
論文番号e99200
ジャーナルPloS one
9
6
DOI
出版ステータス出版済み - 6月 10 2014

!!!All Science Journal Classification (ASJC) codes

  • 一般

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