Ice-water interface migration by temperature controlling for stretching of dna molecules

Jun Komatsu, Michihiko Nakano, Hirofumi Kurita, Kazunori Takashima, Shinji Katsura, Akira Mizuno

研究成果: ジャーナルへの寄稿学術誌査読

3 被引用数 (Scopus)


This report shows a new DNA stretching method using migration of an ice-water interface. DNA molecules were stretched accompanying the migration of the solid-liquid interface and immobilized in frozen area. This simple method needs no chemical modification to keep DNA in the stretched form. For full stretching of DNA molecules, one terminus of the DNA molecules were anchored on silanized substrate. The anchored DNA molecules were stretched by freezing the DNA solution. The stretched DNA molecules were observed after sublimation of the frozen solution keeping its stretched form on silanized surface which had no attractive interaction with DNA molecules except for the SH-modi-fied terminus in solution. An infrared (IR) laser beam was introduced to a frozen DNA solution through an objective lens for local area melting of the solution. Scanning of the laser irradiation caused stretching and enclosing of DNA molecules in the frozen area followed by migration of the solid-liquid interface.

ジャーナルJournal of Biomolecular Structure and Dynamics
出版ステータス出版済み - 12月 2004

!!!All Science Journal Classification (ASJC) codes

  • 構造生物学
  • 分子生物学


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