High sensitive optical detection of bio-chemicals onto a silicon oxide surface based on waveguide mode

Nobuko Fukuda, Makoto Fujimaki, Koichi Awazu, Kaoru Tamada, Kiyoslii Yase

研究成果: 書籍/レポート タイプへの寄稿会議への寄与


This work presents high sensitive detection of specific adsorption of proteins onto a silicon oxide (SiOx) surface modified by molecules with a biotinyl-end group. We fabricated a SiOx waveguide by sputtering onto a thin gold film and measured the incident angle-dependence of reflectivity using a polarized He-Ne laser under total internal reflection condition. When the s-polarized light was irradiated to the SiOx waveguide (760 nm) coupled with a prism, the incident angle-dependence of reflectivity showed narrow dips due to excitation of the waveguide mode. The waveguide surface was modified by the molecules with the biotinyl-end group as a protein probe. Specific adsorption of streptavidin in a PBS buffer solution was monitored through time course of the reflectivity change at a constant incident angle, where the waveguide mode was excited. As compared with conventional SPR system using a gold surface, high sensitive detection of the specific adsorption was achieved. Additionally, we fabricated micro-patterned arrays of biotinyl-end layers onto the SiOx waveguide surface by micro-contact printing (μCP). Then we observed the specific adsorption behavior on the micro-patterned arrays by the imaging based on the waveguide mode using a CCD camera.

ホスト出版物のタイトルNanoparticles and Nanostructures in Sensors and Catalysis
出版社Materials Research Society
ISBN(印刷版)1558998543, 9781558998544
出版ステータス出版済み - 2005
イベント2005 MRS Fall Meeting - Boston, MA, 米国
継続期間: 11月 28 200512月 2 2005


名前Materials Research Society Symposium Proceedings


その他2005 MRS Fall Meeting
CityBoston, MA

!!!All Science Journal Classification (ASJC) codes

  • 材料科学一般
  • 凝縮系物理学
  • 材料力学
  • 機械工学


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