High precursor frequency of human T cells reactive to HLA‐DQ molecules expressed on mouse L cell transfectants

Kazuhiko Fujisawa, Nobuhiro Kamikawaji, Michio Yasunami, Akinori Kimura, Yasuharu Nishimura, Takehiko Sasazuki

研究成果: ジャーナルへの寄稿学術誌査読

8 被引用数 (Scopus)

抄録

In humans, the HLA‐DR molecule is a major stimulatory molecule of allogeneic mixed lymphocyte reactions (MLR) and a major restriction molecule for the presentation of soluble antigens to the T cell. Little is known of the biological function of HLA‐DQ. To examine the size of the repertoire of precursor T cells recognizing the autologous or allogeneic HLA‐DQ molecule, the frequency of T cells reactive to HLA‐DQ was estimated in comparison with T cells reactive to HLA‐DR. We made use of a limiting dilution analysis and mouse L cells transfectants expressing the HLA‐DR or ‐DQ molecule, as stimulators. Human T cells recovered from a primary MLR stimulated with allogeneic peripheral blood lymphocytes (PBL) proliferated in response to L cell transfectants expressing HLA class II genes shared by the stimulator cells in the primary MLR. This observation suggested that the HLA class II molecules on L cell transfectants shared to some extent epitopes for alloreactive T cells with those expressed on human PBL. The precursor frequencies of CD4+ T cells reactive to allogeneic or autologous DQ molecules were as high as those of T cells reactive to allogeneic DR molecules and were estimated to be 1/800 ∼ 1/1800. The frequency of the T cells reactive to autologous DR molecules was low (1/7200 ∼ 1/16000). The biological significance of the high frequency of HLA‐DQ‐reactive precursor T cells is discussed.

本文言語英語
ページ(範囲)2341-2347
ページ数7
ジャーナルEuropean Journal of Immunology
21
10
DOI
出版ステータス出版済み - 10月 1991
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 免疫アレルギー学
  • 免疫学

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