TY - JOUR
T1 - Head and neck cancer antigens recognized by the humoral immune system
AU - Monji, Mikio
AU - Senju, Satoru
AU - Nakatsura, Tetsuya
AU - Yamada, Kazuhiro
AU - Sawatsubashi, Motohiro
AU - Inokuchi, Akira
AU - Nishimura, Yasuharu
N1 - Funding Information:
This work was supported in part by Grants-in-Aid 60715, 11178101, and 12213111 from the Ministry of Education, Science, Technology, Sports, and Culture, Japan.
PY - 2002
Y1 - 2002
N2 - Head and neck cancer in advanced stages are difficult to treat. Therefore development of new treatment modalities and preventive measures are required. We now report the identification of human head and neck cancer antigens recognized by the humoral immune system. We used the serological analysis of recombinant cDNA expression libraries (SEREX) approach. cDNA libraries from cell lines of squamous cell carcinoma of head and neck (SCCHN) and a normal testicle tissue were screened using sera from six allogeneic SCCHN patients. Total 28 positive clones belonging to 19 different genes were identified including 12 known genes and 7 unknown ones. Expression analysis on 13 normal tissues and 13 cancer tissues using reverse transcription-PCR (RT-PCR) revealed eight ubiquitously expressed genes nine of which were expressed preferentially in cancer tissues and two cancer/testis antigens. These antigens we defined may be pertinent candidate antigens for future cancer-diagnosis and related immunotherapy.
AB - Head and neck cancer in advanced stages are difficult to treat. Therefore development of new treatment modalities and preventive measures are required. We now report the identification of human head and neck cancer antigens recognized by the humoral immune system. We used the serological analysis of recombinant cDNA expression libraries (SEREX) approach. cDNA libraries from cell lines of squamous cell carcinoma of head and neck (SCCHN) and a normal testicle tissue were screened using sera from six allogeneic SCCHN patients. Total 28 positive clones belonging to 19 different genes were identified including 12 known genes and 7 unknown ones. Expression analysis on 13 normal tissues and 13 cancer tissues using reverse transcription-PCR (RT-PCR) revealed eight ubiquitously expressed genes nine of which were expressed preferentially in cancer tissues and two cancer/testis antigens. These antigens we defined may be pertinent candidate antigens for future cancer-diagnosis and related immunotherapy.
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U2 - 10.1016/S0006-291X(02)00543-0
DO - 10.1016/S0006-291X(02)00543-0
M3 - Article
C2 - 12056832
AN - SCOPUS:0036293323
SN - 0006-291X
VL - 294
SP - 734
EP - 741
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -
