TY - JOUR
T1 - Glycopolymers Mimicking GM1 Gangliosides
T2 - Cooperativity of Galactose and Neuraminic Acid for Cholera Toxin Recognition
AU - Terada, Yuhei
AU - Hoshino, Yu
AU - Miura, Yoshiko
N1 - Funding Information:
This work was supported by the Japan Agency for Medical Research and Development (JP18fk0108054), Grant-in-Aid for Scientific Research (B) (JP15H03818), Grant-in-Aid for Scientific Research on Innovative Areas (JP18H04420) and Grant-in-Aid for JSPS Fellows (JP17J03810). Victoria Muir, PhD, from Edanz Group (www.edanzediting.com/ac) edited a draft of this manuscript.
Publisher Copyright:
© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2019/4/1
Y1 - 2019/4/1
N2 - Glycopolymers mimicking GM1 gangliosides were synthesized by incorporating multiple types of carbohydrates into the polymer backbone. The glycopolymers were immobilized onto gold surfaces, and the interactions with the cholera toxin B subunit (CTB) were analyzed using surface plasmon resonance imaging. The glycopolymer containing both galactose and neuraminic acid showed enhanced recognition of CTB. The interaction was enhanced mainly because of an improvement in the dissociation process by the binding of the neuraminic acid group in the GM1 binding pocket. This cooperativity of galactose and neuraminic acid was achieved by incorporation into the same flexible polymer backbone, and the importance of the close placement of galactose and neuraminic acid groups was revealed. These results will be valuable in medical fields and also for the development of biofunctional materials.
AB - Glycopolymers mimicking GM1 gangliosides were synthesized by incorporating multiple types of carbohydrates into the polymer backbone. The glycopolymers were immobilized onto gold surfaces, and the interactions with the cholera toxin B subunit (CTB) were analyzed using surface plasmon resonance imaging. The glycopolymer containing both galactose and neuraminic acid showed enhanced recognition of CTB. The interaction was enhanced mainly because of an improvement in the dissociation process by the binding of the neuraminic acid group in the GM1 binding pocket. This cooperativity of galactose and neuraminic acid was achieved by incorporation into the same flexible polymer backbone, and the importance of the close placement of galactose and neuraminic acid groups was revealed. These results will be valuable in medical fields and also for the development of biofunctional materials.
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U2 - 10.1002/asia.201900053
DO - 10.1002/asia.201900053
M3 - Article
C2 - 30809935
AN - SCOPUS:85062787877
SN - 1861-4728
VL - 14
SP - 1021
EP - 1027
JO - Chemistry - An Asian Journal
JF - Chemistry - An Asian Journal
IS - 7
ER -
