Glutathione derivatives enhance adriamycin cytotoxicity in a human lung adenocarcinoma cell line

Yoichi Nakanishi, Hiroaki Matsuki, Koichi Takayama, Jun Yatsunami, Masayuki Kawasaki, Masayoshi Abe, Nobuyuki Hara

研究成果: ジャーナルへの寄稿学術誌査読

8 被引用数 (Scopus)

抄録

We evaluated the effects of a panel of glutathione derivative (S-butyl, S-decyl, S-ethyl, S-heptyl, S-hexyl, S-methyl, S-nonyl, S-octyl, S-propyl and S-pentyl glutathiones) on glutathione-S-transferase activity in the cell lysates of a human lung cancer, PC-9. Glutathione derivatives inhibited glutathione S-transferase activity in PC-9 cell lysates by up to 67%. When PC-9 cells were incubated with the IC50 concentr ation of adriamycin (200 nM) and with nontoxic concentrations (1 μM) of the glutathione derivatives, cytotoxicity ranged from -20% to +55% of the control levels. Enhancement of adriamycin toxicity by glutathione derivatives was significantly correlated with the inhibition of glutathione-S-transferase activity. S-decyl-glutathione, which was one of the most potent inhibitors of glutathione-S-transferase activity, significantly enhanced the adriamycin-induced antitumor effect in vivo. Findings suggest that some glutathione derivatives, including the S-decyl, S-octyl, and S-hexyl glutathiones, enhance adriamycin-induced cytotoxicity, in part by inhibiting glutathione-S-transferase and that these agents may be useful as chemosensitizers for adriamycin therapy. In conclusion, the present results showed that some glutathione derivatives enhanced sensitivity of tumor cells to ADR by inhibiting GST activity. The use of BSO and EA as sensitizers to chemotherapy is currently being evaluated in clinical trials. The present data suggest that the use of GSH derivatives to modulate GST activity may improve the response to ADR.

本文言語英語
ページ(範囲)2129-2134
ページ数6
ジャーナルAnticancer research
17
3 C
出版ステータス出版済み - 1997

!!!All Science Journal Classification (ASJC) codes

  • 腫瘍学
  • 癌研究

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