TY - JOUR
T1 - Genomic organization and chromosomal location of the mouse vasoactive intestinal polypeptide 1 (VPAC1) receptor
AU - Hashimoto, Hitoshi
AU - Nishino, Akiko
AU - Shintani, Norihito
AU - Hagihara, Nami
AU - Copeland, Neal G.
AU - Jenkins, Nancy A.
AU - Yamamoto, Kyohei
AU - Matsuda, Toshio
AU - Ishihara, Takeshi
AU - Nagata, Shigekazu
AU - Baba, Akemichi
N1 - Funding Information:
We gratefully acknowledge Drs. Masayoshi Imagawa and Shige-hiro Osada for valuable discussions and encouragement, Debra J. Gilbert for excellent technical assistance, and Ms. Yukiko Sakagami for secretarial assistance. This research was supported, in part, by the National Cancer Institute, DHHS, under contract with ABL, by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture of Japan, and by grants from Uehara Memorial Foundation and Takeda Science Foundation.
PY - 1999/5/15
Y1 - 1999/5/15
N2 - The gene encoding the mouse vasoactive intestinal polypeptide type 1 (VPAC1) receptor was cloned, and its structural organization was determined. The gene (Vipr1) is more than 16 kh in length and is divided into 13 exons. The 5'-flanking region is highly GC-rich and lacks an apparent TATA box, but contains a CCAAT box, three potential Sp1-binding sites, and two potential AP-2-binding sites. Promoter analysis of the 5'-flanking region of Vipr1 using a luciferase gene reporter system revealed that the isolated 5'- flanking region has functional promoter activity. The mouse Vipr1 gene is encoded by a single gene, which was mapped to the distal region of mouse chromosome 9. This region is syntenic with human chromosome 3p, where the human VPAC1 receptor gene has been mapped.
AB - The gene encoding the mouse vasoactive intestinal polypeptide type 1 (VPAC1) receptor was cloned, and its structural organization was determined. The gene (Vipr1) is more than 16 kh in length and is divided into 13 exons. The 5'-flanking region is highly GC-rich and lacks an apparent TATA box, but contains a CCAAT box, three potential Sp1-binding sites, and two potential AP-2-binding sites. Promoter analysis of the 5'-flanking region of Vipr1 using a luciferase gene reporter system revealed that the isolated 5'- flanking region has functional promoter activity. The mouse Vipr1 gene is encoded by a single gene, which was mapped to the distal region of mouse chromosome 9. This region is syntenic with human chromosome 3p, where the human VPAC1 receptor gene has been mapped.
UR - http://www.scopus.com/inward/record.url?scp=0033563230&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033563230&partnerID=8YFLogxK
U2 - 10.1006/geno.1999.5805
DO - 10.1006/geno.1999.5805
M3 - Article
C2 - 10331949
AN - SCOPUS:0033563230
SN - 0888-7543
VL - 58
SP - 90
EP - 93
JO - Genomics
JF - Genomics
IS - 1
ER -