Genetically fused charged peptides induce rapid crystallization of proteins

K. Minamihata; K. Tsukamoto; M. Adachi; R. Shimizu; M. Mishina; R. Kuroki; T. Nagamune

doi:10.1039/c9cc09529b

Genetically fused charged peptides induce rapid crystallization of proteins

K. Minamihata, K. Tsukamoto, M. Adachi, R. Shimizu, M. Mishina, R. Kuroki, T. Nagamune

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

3 被引用数 (Scopus)

抄録

We utilized electrostatic interaction to induce rapid crystallization of streptavidin. Simply mixing streptavidins possessing either a positively or negatively charged peptide at their C-terminus generated diffraction-quality crystals in a few hours. We modified the streptavidin crystals with fluorescent molecules using biotin, demonstrating the concept of protein crystals as functional biomaterials.

本文言語	英語
ページ（範囲）	3891-3894
ページ数	4
ジャーナル	Chemical Communications
巻	56
号	27
DOI	https://doi.org/10.1039/c9cc09529b
出版ステータス	出版済み - 4月 7 2020

!!!All Science Journal Classification (ASJC) codes

電子材料、光学材料、および磁性材料
セラミックおよび複合材料
金属および合金
材料化学
表面、皮膜および薄膜
化学 (全般)
触媒

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10.1039/c9cc09529b

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abstract = "We utilized electrostatic interaction to induce rapid crystallization of streptavidin. Simply mixing streptavidins possessing either a positively or negatively charged peptide at their C-terminus generated diffraction-quality crystals in a few hours. We modified the streptavidin crystals with fluorescent molecules using biotin, demonstrating the concept of protein crystals as functional biomaterials.",

author = "K. Minamihata and K. Tsukamoto and M. Adachi and R. Shimizu and M. Mishina and R. Kuroki and T. Nagamune",

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AU - Minamihata, K.

AU - Tsukamoto, K.

AU - Adachi, M.

AU - Shimizu, R.

AU - Mishina, M.

AU - Kuroki, R.

AU - Nagamune, T.

PY - 2020/4/7

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AB - We utilized electrostatic interaction to induce rapid crystallization of streptavidin. Simply mixing streptavidins possessing either a positively or negatively charged peptide at their C-terminus generated diffraction-quality crystals in a few hours. We modified the streptavidin crystals with fluorescent molecules using biotin, demonstrating the concept of protein crystals as functional biomaterials.

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Genetically fused charged peptides induce rapid crystallization of proteins

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!!!All Science Journal Classification (ASJC) codes

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