TY - JOUR
T1 - Generation of ovarian follicles from mouse pluripotent stem cells
AU - Yoshino, Takashi
AU - Suzuki, Takahiro
AU - Nagamatsu, Go
AU - Yabukami, Haruka
AU - Ikegaya, Mika
AU - Kishima, Mami
AU - Kita, Haruka
AU - Imamura, Takuya
AU - Nakashima, Kinichi
AU - Nishinakamura, Ryuichi
AU - Tachibana, Makoto
AU - Inoue, Miki
AU - Shima, Yuichi
AU - Morohashi, Ken Ichirou
AU - Hayashi, Katsuhiko
N1 - Publisher Copyright:
© 2021 American Association for the Advancement of Science. All rights reserved.
PY - 2021/7/16
Y1 - 2021/7/16
N2 - Oocytes mature in a specialized fluid-filled sac, the ovarian follicle, which provides signals needed for meiosis and germ cell growth. Methods have been developed to generate functional oocytes from pluripotent stem cell–derived primordial germ cell–like cells (PGCLCs) when placed in culture with embryonic ovarian somatic cells. In this study, we developed culture conditions to recreate the stepwise differentiation process from pluripotent cells to fetal ovarian somatic cell–like cells (FOSLCs). When FOSLCs were aggregated with PGCLCs derived from mouse embryonic stem cells, the PGCLCs entered meiosis to generate functional oocytes capable of fertilization and development to live offspring. Generating functional mouse oocytes in a reconstituted ovarian environment provides a method for in vitro oocyte production and follicle generation for a better understanding of mammalian reproduction.
AB - Oocytes mature in a specialized fluid-filled sac, the ovarian follicle, which provides signals needed for meiosis and germ cell growth. Methods have been developed to generate functional oocytes from pluripotent stem cell–derived primordial germ cell–like cells (PGCLCs) when placed in culture with embryonic ovarian somatic cells. In this study, we developed culture conditions to recreate the stepwise differentiation process from pluripotent cells to fetal ovarian somatic cell–like cells (FOSLCs). When FOSLCs were aggregated with PGCLCs derived from mouse embryonic stem cells, the PGCLCs entered meiosis to generate functional oocytes capable of fertilization and development to live offspring. Generating functional mouse oocytes in a reconstituted ovarian environment provides a method for in vitro oocyte production and follicle generation for a better understanding of mammalian reproduction.
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U2 - 10.1126/science.abe0237
DO - 10.1126/science.abe0237
M3 - Article
C2 - 34437124
AN - SCOPUS:85110450514
SN - 0036-8075
VL - 373
JO - Science
JF - Science
IS - 6552
M1 - eabe0237
ER -