TY - JOUR
T1 - Generation of a monoclonal antibody for INI1/hSNF5/BAF47
AU - Harada, Akihito
AU - Hayashi, Masayasu
AU - Kuniyoshi, Yuuki
AU - Semba, Yuichiro
AU - Sugahara, Satoko
AU - Tachibana, Taro
AU - Ohkawa, Yasuyuki
AU - Fujita, Masatoshi
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2014/2/1
Y1 - 2014/2/1
N2 - INI1/hSNF5/BAF47, which has an SNF5 domain, belongs to the SWI/SNF family. This family is known as ATP-dependent regulators of gene expression by remodeling chromatin structure during cell differentiation. However, the detailed function of INI1/hSNF5/BAF47 is unclear. Here we report the generation of a specific monoclonal antibody for INI1/hSNF5/BAF47 by the mouse iliac lymph node method. The obtained antibody recognized two isoforms of INI1/hSNF5/BAF47 in immunoblotting and precisely recognized the nuclear localization of INI1/hSNF5/BAF47 in immunostaining. This antibody can contribute to further elucidation of the mechanisms of gene expression regulation by INI1/hSNF5/BAF47 during cell differentiation.
AB - INI1/hSNF5/BAF47, which has an SNF5 domain, belongs to the SWI/SNF family. This family is known as ATP-dependent regulators of gene expression by remodeling chromatin structure during cell differentiation. However, the detailed function of INI1/hSNF5/BAF47 is unclear. Here we report the generation of a specific monoclonal antibody for INI1/hSNF5/BAF47 by the mouse iliac lymph node method. The obtained antibody recognized two isoforms of INI1/hSNF5/BAF47 in immunoblotting and precisely recognized the nuclear localization of INI1/hSNF5/BAF47 in immunostaining. This antibody can contribute to further elucidation of the mechanisms of gene expression regulation by INI1/hSNF5/BAF47 during cell differentiation.
UR - http://www.scopus.com/inward/record.url?scp=84894557345&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84894557345&partnerID=8YFLogxK
U2 - 10.1089/mab.2013.0065
DO - 10.1089/mab.2013.0065
M3 - Article
C2 - 24555937
AN - SCOPUS:84894557345
SN - 2167-9436
VL - 33
SP - 49
EP - 51
JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
IS - 1
ER -