Gene transfer of inhibitor KappaB in human lung cancer cell line NCI-H460 inhibits tumorigenesis and angiogenesis in vivo

Background: Nuclear factor kappaB (NFκB) is an inducible and ubiquitously expressed transcription factor which is involved in cell survival, differentiation and growth and, thus, has also been implicated in tumor formation and development. Research on the effect of NFκB in inhibiting cancer cell growth, however, remains controversial. Materials and Methods: We investigated the effects of overexpressed IκBα on the proliferation of the human lung cancer cell line H460 in vitro and in vivo using IκBα-expressing adenovirus. Results: The results suggested that the infection of AdIκBα blocked NFαB activity in H460 cells and significantly inhibited cell proliferation by inducing apoptosis. An in vivo study showed the tumor incidence to be significantly lower in mice implanted with H460 cells infected with AdIκBα. For established H460 tumor, the intratumoral injection of AdIκBα also inhibited the tumor growth due to both a blockade of the NFκB activity and an inhibition of the VEGF expression. Conclusion: Adenovirus-mediated IκBα gene transfer is a promising cancer treatment strategy.