Gene expression during the development of experimentally induced cerebral aneurysms

Nobutake Sadamasa, Kazuhiko Nozaki, Hiroko Kita-Matsuo, Sakae Saito, Takuya Moriwaki, Tomohiro Aoki, Satoshi Kawarazaki, Hiroharu Kataoka, Yasushi Takagi, Masatsune Ishikawa, Nobuo Hashimoto, Kikuya Kato

研究成果: ジャーナルへの寄稿学術誌査読

11 被引用数 (Scopus)

抄録

Cerebral aneurysms are a major cause of subarachnoid hemorrhage, but the mechanism of their development remains unclear. In this study, we investigated candidate genes whose expression is significantly changed during the development of experimentally induced cerebral aneurysms using adaptor-tagged competitive polymerase chain reaction (ATAC-PCR). Twenty-four rats received sham operation (control) or the operation for the induction of experimental cerebral aneurysms. Rats were sacrificed at time 0, 2 weeks, 1 month and 3 months after the operation (n = 6 for each group). RNAs from right anterior cerebral artery/olfactory artery (ACA/OA) bifurcations were assessed via a 191-gene data matrix expression profile by ATAC-PCR. We identified 15 genes whose expression is significantly altered during cerebral aneurysm formation, including major heparan sulfate proteoglycan, cathepsin B, hevin and β4- integrin. We also confirmed protein expression of β4-integrin in rat cerebral aneurysms by quantitative real-time PCR and immunohistochemistry. The ATAC-PCR revealed temporal changes in gene expression during the development of experimental cerebral aneurysms. The genes that were significantly changed in this study would be the candidates for future studies concerning the development of cerebral aneurysms.

本文言語英語
ページ(範囲)343-349
ページ数7
ジャーナルJournal of Vascular Research
45
4
DOI
出版ステータス出版済み - 6月 2008
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生理学
  • 循環器および心血管医学

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