Gadolinium blocks mechano-electric transducer current in chick cochlear hair cells

Takashi Kimitsuki; Takashi Nakagawa; Kazutaka Hisashi; Shizuo Komune; Sohtaro Komiyama

doi:10.1016/S0378-5955(96)00134-7

Gadolinium blocks mechano-electric transducer current in chick cochlear hair cells

Takashi Kimitsuki
, Takashi Nakagawa
, Kazutaka Hisashi
, Shizuo Komune
, Sohtaro Komiyama

外科学

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

40 被引用数 (Scopus)

抄録

We investigated the effects of gadolinium ion (Gd³⁺) on the mechano-electrical transduction (MET) current using a whole-cell patch electrode voltage clamp technique in dissociated cochlear hair cells of chicks. Gd³⁺ blocked the MET channel in a concentration- and voltage-dependent manner. At -50 mV, Gd³⁺ blocked the MET channel, with a Hill coefficient of 1.14 and a dissociation constant (K(D)) of 1.01 x 10^-5 M. Adaptation of the MET current disappeared after the introduction of Gd³⁺, a change that may be due to a decrease in inward going MET currents, specifically the Ca²⁺ component.

本文言語	英語
ページ（範囲）	75-80
ページ数	6
ジャーナル	Hearing Research
巻	101
号	1-2
DOI	https://doi.org/10.1016/S0378-5955(96)00134-7
出版ステータス	出版済み - 11月 1996

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10.1016/S0378-5955(96)00134-7

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@article{f966556d851d483990ae53de1847f0b7,

title = "Gadolinium blocks mechano-electric transducer current in chick cochlear hair cells",

abstract = "We investigated the effects of gadolinium ion (Gd3+) on the mechano-electrical transduction (MET) current using a whole-cell patch electrode voltage clamp technique in dissociated cochlear hair cells of chicks. Gd3+ blocked the MET channel in a concentration- and voltage-dependent manner. At -50 mV, Gd3+ blocked the MET channel, with a Hill coefficient of 1.14 and a dissociation constant (K(D)) of 1.01 x 10-5 M. Adaptation of the MET current disappeared after the introduction of Gd3+, a change that may be due to a decrease in inward going MET currents, specifically the Ca2+ component.",

author = "Takashi Kimitsuki and Takashi Nakagawa and Kazutaka Hisashi and Shizuo Komune and Sohtaro Komiyama",

note = "Funding Information: The authors appreciate the technical support of Mr. T. Shioya. This work was supported by a Grant-in-Aid (03454406) from the Ministry of Education, Science and Culture of Japan.",

year = "1996",

month = nov,

doi = "10.1016/S0378-5955(96)00134-7",

language = "English",

volume = "101",

pages = "75--80",

journal = "Hearing Research",

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TY - JOUR

T1 - Gadolinium blocks mechano-electric transducer current in chick cochlear hair cells

AU - Kimitsuki, Takashi

AU - Nakagawa, Takashi

AU - Hisashi, Kazutaka

AU - Komune, Shizuo

AU - Komiyama, Sohtaro

N1 - Funding Information: The authors appreciate the technical support of Mr. T. Shioya. This work was supported by a Grant-in-Aid (03454406) from the Ministry of Education, Science and Culture of Japan.

PY - 1996/11

Y1 - 1996/11

N2 - We investigated the effects of gadolinium ion (Gd3+) on the mechano-electrical transduction (MET) current using a whole-cell patch electrode voltage clamp technique in dissociated cochlear hair cells of chicks. Gd3+ blocked the MET channel in a concentration- and voltage-dependent manner. At -50 mV, Gd3+ blocked the MET channel, with a Hill coefficient of 1.14 and a dissociation constant (K(D)) of 1.01 x 10-5 M. Adaptation of the MET current disappeared after the introduction of Gd3+, a change that may be due to a decrease in inward going MET currents, specifically the Ca2+ component.

AB - We investigated the effects of gadolinium ion (Gd3+) on the mechano-electrical transduction (MET) current using a whole-cell patch electrode voltage clamp technique in dissociated cochlear hair cells of chicks. Gd3+ blocked the MET channel in a concentration- and voltage-dependent manner. At -50 mV, Gd3+ blocked the MET channel, with a Hill coefficient of 1.14 and a dissociation constant (K(D)) of 1.01 x 10-5 M. Adaptation of the MET current disappeared after the introduction of Gd3+, a change that may be due to a decrease in inward going MET currents, specifically the Ca2+ component.

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U2 - 10.1016/S0378-5955(96)00134-7

DO - 10.1016/S0378-5955(96)00134-7

M3 - Article

C2 - 8951434

AN - SCOPUS:0030297569

SN - 0378-5955

VL - 101

SP - 75

EP - 80

JO - Hearing Research

JF - Hearing Research

IS - 1-2

ER -

Gadolinium blocks mechano-electric transducer current in chick cochlear hair cells

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!!!All Science Journal Classification (ASJC) codes

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