TY - JOUR
T1 - Functional expression and tissue distribution of a novel receptor for vasoactive intestinal polypeptide
AU - Ishihara, Takeshi
AU - Shigemoto, Ryuichi
AU - Mori, Kensaku
AU - Takahashi, Kenji
AU - Nagata, Shigekazu
N1 - Funding Information:
We thank Drs. R. Yoshida, K. Katoh, and K. lmamura for help with the in situ hybridization, Dr. M. Nishizawa for discussion, and Ms. M. lkeda for secretarial assistance. This work was supported in part by a Grant-in-Aid from the Ministry of Education, Science and Culture of Japan.
PY - 1992/4
Y1 - 1992/4
N2 - Vasoactive intestinal polypeptide (VIP), a 28 amino acid peptide hormone, plays many physiological roles in the peripheral and central nerve systems. A functional cDNA clone of the VIP receptor was isolated from a rat lung cDNA library by cross-hybridization with the secretin receptor cDNA. VIP bound the cloned VIP receptor expressed in mouse COP cells and stimulated adenylate cyclase through the cloned receptor. The rat VIP receptor consists of 459 amino acids with a calculated Mr of 52,054 and contains seven transmembrane segments. It is structurally related to the secretin, calcitonin, and parathyroid hormone receptors, suggesting that they constitute a new subfamily of the Gs protein-coupled receptors. VIP receptor mRNA was detected in various rat tissues including liver, lung, intestines, and brain. In situ hybridization revealed that VIP receptor mRNA is widely distributed in neuronal cells of the adult rat brain, with a relatively high expression in the cerebral cortex and hippocampus.
AB - Vasoactive intestinal polypeptide (VIP), a 28 amino acid peptide hormone, plays many physiological roles in the peripheral and central nerve systems. A functional cDNA clone of the VIP receptor was isolated from a rat lung cDNA library by cross-hybridization with the secretin receptor cDNA. VIP bound the cloned VIP receptor expressed in mouse COP cells and stimulated adenylate cyclase through the cloned receptor. The rat VIP receptor consists of 459 amino acids with a calculated Mr of 52,054 and contains seven transmembrane segments. It is structurally related to the secretin, calcitonin, and parathyroid hormone receptors, suggesting that they constitute a new subfamily of the Gs protein-coupled receptors. VIP receptor mRNA was detected in various rat tissues including liver, lung, intestines, and brain. In situ hybridization revealed that VIP receptor mRNA is widely distributed in neuronal cells of the adult rat brain, with a relatively high expression in the cerebral cortex and hippocampus.
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U2 - 10.1016/0896-6273(92)90101-I
DO - 10.1016/0896-6273(92)90101-I
M3 - Article
C2 - 1314625
AN - SCOPUS:0026511295
SN - 0896-6273
VL - 8
SP - 811
EP - 819
JO - Neuron
JF - Neuron
IS - 4
ER -