TY - JOUR
T1 - Fractionation of mammalian tissue mRNAs by high-performance gel filtration chromatography
AU - Ogishima, Tadashi
AU - Okada, Yoshiie
AU - Omura, Tsuneo
PY - 1984/5/1
Y1 - 1984/5/1
N2 - Total RNA was prepared from the free polysomes of bovine adrenal cortex and fractionated by high-performance gel filtration chromatography using a TSK-GEL G4000SW or G5000PW column. The former column gave better separation of 28 and 18 S rRNA than did the latter column when a suitable elution condition was selected. The separation of mRNAs by gel filtration chromatography was examined by in vitro translation of the fractionated RNA samples, and the fractionation of mRNAs roughly according to their sizes was confirmed. Significant enrichment of the mRNA of cytochrome P-450(SCC), whose in vitro-synthesized peptide had a molecular weight of 55,000, was achieved.
AB - Total RNA was prepared from the free polysomes of bovine adrenal cortex and fractionated by high-performance gel filtration chromatography using a TSK-GEL G4000SW or G5000PW column. The former column gave better separation of 28 and 18 S rRNA than did the latter column when a suitable elution condition was selected. The separation of mRNAs by gel filtration chromatography was examined by in vitro translation of the fractionated RNA samples, and the fractionation of mRNAs roughly according to their sizes was confirmed. Significant enrichment of the mRNA of cytochrome P-450(SCC), whose in vitro-synthesized peptide had a molecular weight of 55,000, was achieved.
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U2 - 10.1016/0003-2697(84)90813-3
DO - 10.1016/0003-2697(84)90813-3
M3 - Article
C2 - 6742407
AN - SCOPUS:0021431156
SN - 0003-2697
VL - 138
SP - 309
EP - 313
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -