Formation of aberrant TJ strands by overexpression of claudin-15 in MDCK II cells

Akihito Sengoku, Tetsuichiro Inai, Yosaburo Shibata

研究成果: ジャーナルへの寄稿学術誌査読

7 被引用数 (Scopus)


Claudins are one of the transmembrane proteins found at tight junctions (TJs); they constitute the backbone of TJ strands and comprise a multigene family. Claudins share a YV sequence at the COOH-termini, which is considered to be a ZO-binding motif. Overexpression of claudin-15 (15CL) or claudin-15 tagged with enhanced green fluorescent protein at the NH2-terminus (EGFP-15CL) induced aberrant strands in MDCK II cells, even though claudin-15 has the ZO-binding motif. Morphometric analysis by freeze-fracture electron microscopy revealed that the mean number of apical TJ strands increased by 47% in EGFP-1CL-expressing cells, 21% in EGFP-15CL-expressing cells, and 28% in 15CL-expressing cells. The number of free-ended apical strands increased remarkably in EGFP-15CL- and 15CL-expressing cells, but not in EGFP-1CL-expressing cells. When MDCK cells expressing EGFP-1CL, EGFP-15CL or 15CL were co-cultured with parent MDCK cells, which express claudin-1 but not claudin-15, EGFP-15CL and 15CL could not be concentrated at the apical junctional region between the heterotypic cells, though EGFP-1CL could. These results suggest that not only binding to ZO-1, but also head-to-head compatibility between claudin species, is involved in organizing claudin proteins at the apical junctional region.

ジャーナルHistochemistry and Cell Biology
出版ステータス出版済み - 2月 2008

!!!All Science Journal Classification (ASJC) codes

  • 組織学
  • 分子生物学
  • 医療検査技術
  • 細胞生物学


「Formation of aberrant TJ strands by overexpression of claudin-15 in MDCK II cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。