Fluorescent indicator displacement assay of ligands targeting 10 microRNA precursors

Asako Murata, Yasue Harada, Takeo Fukuzumi, Kazuhiko Nakatani

研究成果: ジャーナルへの寄稿学術誌査読

21 被引用数 (Scopus)

抄録

Fluorescent indicator displacement (FID) assay is a rapid and convenient assay for identifying new ligands that bind to the target molecules. In our previous studies, we have shown that a series of 2,7-diaminoalkoxy xanthone and thioxanthone derivatives can be used as fluorescent indicators for detecting the interaction between RNA and a ligand. The xanthone and thioxanthone fluorochromes showed efficient fluorescence quenching upon binding to target RNA. Subsequent displacement of the bound-fluorochrome with a ligand that binds more strongly to the target RNA led to the recovery of the fluorescence by releasing the fluorochrome from RNA. Here we report a pilot screening of a chemical library that contains 9600 structurally diverse compounds for molecules that bind to a specific miRNA precursor using the FID assay.

本文言語英語
ページ(範囲)7101-7106
ページ数6
ジャーナルBioorganic and Medicinal Chemistry
21
22
DOI
出版ステータス出版済み - 11月 15 2013
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子医療
  • 分子生物学
  • 薬科学
  • 創薬
  • 臨床生化学
  • 有機化学

フィンガープリント

「Fluorescent indicator displacement assay of ligands targeting 10 microRNA precursors」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル