Fluorescent detection of target proteins: Via a molecularly imprinted hydrogel

Takuya Kubo, Naoki Watanabe, Seiji Ikari, Chenchen Liu, Eisuke Kanao, Toyohiro Naito, Tomoharu Sano, Koji Otsuka

研究成果: ジャーナルへの寄稿学術誌査読

4 被引用数 (Scopus)


Proteins are typically separated by an immune reaction, such as an enzyme-linked immunosorbent assay, and are detected by selective fluorescent labeling. This has potential for complicated procedures and the denaturation of proteins by labeling, and is cost consuming. In this study, we propose a technique for the selective separation and detection of a target protein using a molecularly imprinted hydrogel (PI gel) with fluorescent monomers. We focused on 8-anilino-1-naphthalenesulfonic acid (ANS), where the fluorescence intensity is easily changed by the interaction with proteins, and successfully synthesized the ANS monomer and a poly(ethylene glycol) (PEG) conjugated ANS monomer. The PI gel with the ANS monomers using bovine serum albumin (BSA) as a template showed the selective adsorption of BSA and the fluorescence intensity increased due to the adsorption of BSA.

ジャーナルAnalytical Methods
出版ステータス出版済み - 7月 21 2021

!!!All Science Journal Classification (ASJC) codes

  • 分析化学
  • 化学工学一般
  • 工学一般


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