Fabrication and evaluation of microfluidic immunoassay devices with antibody-immobilized microbeads retained in porous hydrogel micropillars

Toshihiro Kasama, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

研究成果: 書籍/レポート タイプへの寄稿

3 被引用数 (Scopus)

抄録

Due to the inherent characteristics including confinement of molecular diffusion and high surface-to-volume ratio, microfluidic device-based immunoassay has great advantages in cost, speed, sensitivity, and so on, compared with conventional techniques such as microtiter plate-based ELISA, latex agglutination method, and lateral flow immunochromatography. In this paper, we explain the detection of C-reactive protein as a model antigen by using our microfluidic immunoassay device, so-called immuno-pillar device. We describe in detail how we fabricated and used the immuno-pillar devices.

本文言語英語
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ49-56
ページ数8
DOI
出版ステータス出版済み - 2017
外部発表はい

出版物シリーズ

名前Methods in Molecular Biology
1547
ISSN(印刷版)1064-3745

!!!All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 遺伝学

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